The effect of tumor necrosis factor alpha on human trophoblast cells

Impaired trophoblast cell invasion is believed to be an inciting factor in preeclampsia. Factors driving are incompletely understood. Maternal serum tumor necrosis alpha (TNFα) levels elevated We hypothesized that TNFα influences human cells. treated immortalized first trimester cells (HTR-8/SVneo) with 10 or 100ng/ml for 4 24 hrs. measured protein of soluble fms like tyrosine kinase 1 (sFLT1) the supernatant culture by enzyme-linked immunosorbent assay (ELISA), and transcription sFLT1 EDN1 (gene coding endothelin-1, ET-1) qRT-PCR. After hrs treatment, at doses 10, 100 ng/ml significantly increased compared controls (panel A & B Fig.). hrs, mRNAs were higher lower while mRNA not different from those C after treatment E exposure induces production anti-angiogenic ET-1. may execute feedback inhibition on itself. The next step determine effect invasion.